Histological Changes of Diaphyse of Tibia and its Central Medulary Chanal Follwing Low-Power Laser Irradiation in Rabbit

Purpose: The reaction of compact bone of diaphyse of tibia and it's bone marrow to low power laser radiation was investigated. Materials and Methods: Ten adult male rabbits were divided randomly into experimental and control groups. Low-power He-Ne laser (36 J/cm2) were radiated into a fixed point of tibia daily for 14 consequative days. Rabbits of control group did not receive laser. At the end of the period rabbits were killed by chloroform and a sample from each rabbit was extracted. Samples were fixed in formalin saline and were decalcified by EDTA, and prepared for light microscopic study. Sections were stained by Hematoxyline and Eosine method and examined descriptively and quantitatively. Results: In control group, compact bone is composed of lamellar bone which includes many regular haversian systems. In experimental group lamellae of haversian systems were irregular and in some of haversian chanals, hemopoitic activities were observed. Periosteum of experimental group was thicker than control group. There were more blood cells in the central medulary chanal of experimental group than control group. Conclusion: He-Ne low-power laser radiation increased periosteal thickness and hemopoitic activity of rabbit's Tibia.